3216 rrid cvcl 0063 Search Results


hek 293t cells  (ATCC)
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cell lines  (ATCC)
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crl 3216 rrid cvcl 0063 recombinant dna reagent phage cdkn2a plasmid  (Addgene inc)
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Addgene inc crl 3216 rrid cvcl 0063 recombinant dna reagent phage cdkn2a plasmid
Figure 2. Functional characterization of all possible <t>CDKN2A</t> missense variants. (A) Functional classifications for 3120 CDKN2A variants, including 2964 missense variants and 156 synonymous variants. Variants were classified as functionally deleterious, indeterminate function, or neutral based on p-value using gamma generalized linear model (GLM). 525 (17.7%) variants were classified as functionally deleterious. (B) Log2 p-value (gamma GLM) for 32 benchmark pathogenic variants, 6 benign variants, 31 variants of uncertain significance (VUSs) previously reported to have functionally deleterious
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rrid hbec3 kt atcc crl 4051 rrid cvcl x491 mle12 atcc crl 2110 rrid cvcl 3751 a549 atcc ccl 185 rrid cvcl 0023 293t atcc crl 3216 rrid cvcl 0063 tools  (ATCC)
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ATCC rrid hbec3 kt atcc crl 4051 rrid cvcl x491 mle12 atcc crl 2110 rrid cvcl 3751 a549 atcc ccl 185 rrid cvcl 0023 293t atcc crl 3216 rrid cvcl 0063 tools
Figure 2. Functional characterization of all possible <t>CDKN2A</t> missense variants. (A) Functional classifications for 3120 CDKN2A variants, including 2964 missense variants and 156 synonymous variants. Variants were classified as functionally deleterious, indeterminate function, or neutral based on p-value using gamma generalized linear model (GLM). 525 (17.7%) variants were classified as functionally deleterious. (B) Log2 p-value (gamma GLM) for 32 benchmark pathogenic variants, 6 benign variants, 31 variants of uncertain significance (VUSs) previously reported to have functionally deleterious
Rrid Hbec3 Kt Atcc Crl 4051 Rrid Cvcl X491 Mle12 Atcc Crl 2110 Rrid Cvcl 3751 A549 Atcc Ccl 185 Rrid Cvcl 0023 293t Atcc Crl 3216 Rrid Cvcl 0063 Tools, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neaa  (Thermo Fisher)
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Thermo Fisher neaa
Figure 2. Functional characterization of all possible <t>CDKN2A</t> missense variants. (A) Functional classifications for 3120 CDKN2A variants, including 2964 missense variants and 156 synonymous variants. Variants were classified as functionally deleterious, indeterminate function, or neutral based on p-value using gamma generalized linear model (GLM). 525 (17.7%) variants were classified as functionally deleterious. (B) Log2 p-value (gamma GLM) for 32 benchmark pathogenic variants, 6 benign variants, 31 variants of uncertain significance (VUSs) previously reported to have functionally deleterious
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glutamax  (Thermo Fisher)
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Thermo Fisher glutamax
Figure 2. Functional characterization of all possible <t>CDKN2A</t> missense variants. (A) Functional classifications for 3120 CDKN2A variants, including 2964 missense variants and 156 synonymous variants. Variants were classified as functionally deleterious, indeterminate function, or neutral based on p-value using gamma generalized linear model (GLM). 525 (17.7%) variants were classified as functionally deleterious. (B) Log2 p-value (gamma GLM) for 32 benchmark pathogenic variants, 6 benign variants, 31 variants of uncertain significance (VUSs) previously reported to have functionally deleterious
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Image Search Results


Key Resources

Journal: Neuron

Article Title: Endogenous Piezo1 can confound mechanically-activated channel identification and characterization

doi: 10.1016/j.neuron.2017.03.039

Figure Lengend Snippet: Key Resources

Article Snippet: Method Details Cell culture and transfection Both HEK293T (“HEK”; DSMZ Cat# ACC-635, RRID:CVCL_0063 and ATCC Cat# CRL-3216) and HEK-P1KO cell lines were maintained in DMEM (high glucose) with 10% FBS and pen/strep antibiotics.

Techniques: Virus, Recombinant, Plasmid Preparation, Software

Figure 2. Functional characterization of all possible CDKN2A missense variants. (A) Functional classifications for 3120 CDKN2A variants, including 2964 missense variants and 156 synonymous variants. Variants were classified as functionally deleterious, indeterminate function, or neutral based on p-value using gamma generalized linear model (GLM). 525 (17.7%) variants were classified as functionally deleterious. (B) Log2 p-value (gamma GLM) for 32 benchmark pathogenic variants, 6 benign variants, 31 variants of uncertain significance (VUSs) previously reported to have functionally deleterious

Journal: eLife

Article Title: Functional characterization of all CDKN2A missense variants and comparison to in silico models of pathogenicity

doi: 10.7554/elife.95347

Figure Lengend Snippet: Figure 2. Functional characterization of all possible CDKN2A missense variants. (A) Functional classifications for 3120 CDKN2A variants, including 2964 missense variants and 156 synonymous variants. Variants were classified as functionally deleterious, indeterminate function, or neutral based on p-value using gamma generalized linear model (GLM). 525 (17.7%) variants were classified as functionally deleterious. (B) Log2 p-value (gamma GLM) for 32 benchmark pathogenic variants, 6 benign variants, 31 variants of uncertain significance (VUSs) previously reported to have functionally deleterious

Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CDKN2A GenBank Gene ID: 1029 NM_000077.5 NP_000068.1 Cell line (H. sapiens) PANC- 1 American Type Culture Collection Cat. #: CRL- 1469 RRID:CVCL_0480 Cell line (H. sapiens) 293T American Type Culture Collection Cat. #: CRL- 3216 RRID:CVCL_0063 Recombinant DNA reagent pHAGE- CDKN2A (plasmid) Addgene RRID:Addgene_116726 Lentiviral vector expressing CDKN2A Recombinant DNA reagent pLentiV_ Blast (plasmid) Addgene RRID:Addgene_111887 Recombinant DNA reagent pLJM1- Empty (plasmid) Addgene RRID:Addgene_91980 Recombinant DNA reagent psPAX2 (plasmid) Addgene RRID:Addgene_12260 Recombinant DNA reagent pCMV- VSV- G (plasmid) Addgene RRID:Addgene_8454 Recombinant DNA reagent pLJM1- CDKN2A (plasmid) Twist Bioscience Lentiviral vector expressing codon- optimized CDKN2A Recombinant DNA reagent pLJM1- CDKN2A- Leu32Leu (plasmid) This paper Lentiviral vector expressing CDKN2A- Leu32Leu Recombinant DNA reagent pLJM1- CDKN2A- Leu32Pro (plasmid) This paper Lentiviral vector expressing CDKN2ALeu32Pro Recombinant DNA reagent pLJM1- CDKN2A- Gly101Gly (plasmid) This paper Lentiviral vector expressing CDKN2AGly101Gly Recombinant DNA reagent pLJM1- CDKN2A- Gly101Trp (plasmid) This paper Lentiviral vector expressing CDKN2AGly101Trp Recombinant DNA reagent pLJM1- CDKN2A- Val126Asp (plasmid) This paper Lentiviral vector expressing CDKN2AVal126Asp Recombinant DNA reagent pLJM1- CDKN2A- Val126Val (plasmid) This paper Lentiviral vector expressing CDKN2AVal126Val Kimura et al. eLife 2024;13:RP95347.

Techniques: Functional Assay